EVALUATION OF METHYL TERT-BUTYL ETHER (MTBE)-INDUCED DNA DAMAGE IN ISOLATED RAT SPERMATOGENIC CELLS
Methyl ten-butyl ether (MTBE) is oxygenate hydrocarbon. In the United States MTBE pollution is regarded as a serious environmental problem. The objective of the present study was to investigate oxidative stress and DNA damage induced by MTBE and to investigate relationship between oxidative stress and DNA damage in isolated rat spermatogenic cells, Spermatogenic cells isolated from testes of adult Sprague-Dawley rats by a mechanical procedure without the use of trypsin In study of MTBE-induced oxidative stress, ROS production was tested using a 2, 7- Dichlorofluorescein diacetate (DCHF-DA) probe. Cells were incubated with medium alone (control). 0. 5, 5, 50 mM MTBE. respectively, for 1, 2, 3, 6 hours. In study of DNA damage. Alkaline single-cell gel electrophoresis CSCGE) assays was used. Cells were incubated with medium alone (control), 0. 5, 5, 50 mM MTBE, respectively, for 2 and 4 hours. The results showed that MTBE significantly increased reactive oxygen species (ROS) production for 1, 2, 3 h and DNA damage for 2 h (P＜0. 05 or P＜0. 01). For 4 h, only exposure of spermatogenic cells to 50 mM has significantly increased the percentage of both damaged and strongly damaged spots compared to control (P＜0. 01). Our results suggest that MTBE induced DNA damage by increasing ROS production, and this DNA oxidative damage can be repaired by repair system of spermatogenic cells.
MTBE SCGE DNA damage oxidative stress spermatogenic cells
Dongmei Li Xiaodong Han
Immunology and Reproductive Biology Laboratory, Medical School, Nanjing University, Nanjing 210093, China
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
483-487
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）