EXPRESSION AND ALTERATION OF INSULIN-LIKE GROWTH FACTOR Ⅱ-MESSENGER RNA IN HEPATOMA TISSUES AND PERIPHERAL BLOOD OF HCC PATIENTS
The prognosis of hepatocellular carcinoma (HCC) still remains dismal Insulin-like growth factor Ⅱ (IGF - Ⅱ ) is a fetal growth factor and highly expressed during HCC development. In the present study, the clinical values of serum free IGF - Ⅱ levels and IGF - Ⅱ mRNA in HCC tissues and peripheral blood were investigated in diagnosis of HCC and monitoring of extrahepatic metastasis. The amplified fragments of IGF - Ⅱ mRNA by RT-PCR were identical to original designed ones with size of 170 bp and confirmed by sequencing analysis. The dilution experiments revealed that the lowest sensitivity of our system was 2 ng/L of total RNA. The positive frequencies of IGF - Ⅱ mRNA were 100％ in HCC tissues, 53. 3％ in paracancerous tissues, and none in noncancerous tissues, respectively. The serum free IGF - Ⅱ levels were significantly higher in HCC than those in chronic hepatitis or liver cirrhosis. The frequency of circulating IGF - Ⅱ mRNA was 34. 2％ in HCC. no fragment was found in other liver diseases, extrahepatic tumors, and normal controls, respectively. The circulating IGF - Ⅱ mRNA correlated with the stage of HCC. and its positive rate was 100％ in HCC with extrahepatic metastasis and 35. 5％ in HCC widi AFP-negative. No significant difference was found between tumor sizes and circulating IGF -Ⅱ mRNA. The abnormal expression of free IGF -Ⅱ and IGF -Ⅱ mRNA are useful tumor markers for HCC diagnosis, differentiation of extrahepatic metastasis and monitoring postoperative recurrence.
HCC IGF- Ⅱ -mRNA RT-PCR diagnosis sequence
Lei Zong Dengfu Yao Zhizheng Dong Xinhua Wu Wei Wu Xianyong Meng
Research Center of Clinical Molecular Biology, Affiliated Hospital of Nantong University, Nantong 22 Department of Hematology, Affiliated Hospital of Nantong University, Nantong 226001, China Department of Gastroenterology, Affiliated Hospital of Nantong University,Nantong 226001, China
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
488-491
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）