FAS PATHWAY IN APOPTOSIS OF MOUSE PERITONEAL MACROPHAGES INDUCED BY NONYLPHENOL
Nonylphenol (NP) is the final biodegradation product of nonylphenol polyethoxylates(NPEs), which are widely used as surfactants in domestic and industrial products. NP has been reported to have potential reproductive toxicity. However, its influence on immune system function remains unclear. In this studyt to determine the immunological effects of NP, the effects of NP on apoptosis and Fas/FasL gene expression in mouse peritoneal macrophages(MPM) in vitro were investigated. MPMs were treated with NP2, 4, 8 mg/mL respectively. The apoptosis of MPM was evaluated by flow cytometry analysis system(FCAS). A semi-quantitative reverse transcriptase-polymerase chain reaction method was used to analyze Fas and FasL mRNA levels. The results showed that NP induced apoptosis and enhanced the expression of Fas and FasL mRNA of MPM. There are dose-effect relationships in these effects. These findings suggest that NP may induce apoptosis by altering the expression of Fas and FasL in MPM so as to affect the immune system.
Nonylphenol mouse peritoneal macrophages apoptosis Fas/FasL
Sunan Shen Yanfei Xu Genhong Yao Xiaodong Han
Immunology and reproductive biology Laboratory, Medical School, Nanjing University,Nanjing 210093, China
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
498-501
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）