Determination of glucose and albumin in urine using Mid-Infrared Attenuated Total Reflection System

摘要：

A reagent-free method applied for component analysis of glucose and albumin in urine which using ATR spectroscopy was studied. Concentration of glucose and albumin have significant mid-infrared absorption bands. They are relate to the molecule vibration of CH, NH, CO or CC. The calibration model of glucose and albumin in urine have been established using attenuated total reflectance (ATR) Mid-infrared spectroscopy combined with partial least squares (PLS) regression. Compared with biochemical method, this method has provided with advantages such as high- speed, simplicity and good repeatability. RMSEP (Root Mean Square Error of Prediction) for glucose and albumin is 20.5 mg/dL and 17.8 mg/dL respectively, and the correlation is 0.999 and 0.998. The result method have the potential to serve as an effective addition to the clinical chemistry laboratory.

关键词： components in urine Mid-Infrared spectroscopy ATR Partial Least Squares (PLS)

作者: Weiling Liu Libing Liu Kexin Xu Qilian Yu

作者单位: Hebei University of Technology, Dizigu Road, Hongqiao District, Tianjin 300130 China Tianjin University, Weijin Road, Nankai District, Tianjin 300072, China

会议类型: 国际会议

会议名称: 2006 IEEE International Conference on Information Acquisition

会议地点: 山东威海

会议语种:英文

页码: 1248-1252

在线出版日期: 2006-08-20（万方平台首次上网日期，不代表论文的发表时间）