Safety of inoculation of bovine parainfluenza virus 3 as potential vaccine vector in pigs
Bovine parainfluenza virus 3 (BPIV3) is one of the most important viral respiratory pathogens in cattle.One BPIV3,named NM09,was isolated from cattle suffering from severe respiratory diseases in 2009.BPIV3 is a potential recombinant vaccine vector.To investigate whether NM09 can infect pigs and determine BPIV3 defense in these animals,BPIV3 antibody-free pigs were inoculated intramuscularly with the BPIV3 NM09 strain in a continuous passage.Clinical signs were observed each day after inoculation.Viral nucleic acid was detected in nasal and anal secretions.Results showed that virus-inoculated pigs displayed few observable clinical signs related to respiratory diseases.The antibody was identified,but the virus could not be detected in the second continuous passage in pigs.Thus,BPIV3 is a potential vaccine vector for genetic engineering.
bovine parainfluenza virus potential vaccine vector safety
Feng-Xue Wang Ying Liu Hong-wei Zhu Xing Liu Yong Yang Na Sun Shi-Peng Cheng Yong-Jun Wen
State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, No.4899 Juye Avenue, Jingyue Economic and Technological Development Zone, Changchun, Jilin 130112, P.R.China
国内会议
拉萨
英文
200-205
2015-08-01（万方平台首次上网日期，不代表论文的发表时间）