Effects of silencing TPX2 by siRNA on radiotherapeutic sensitivity of Hela cell in Human cervical Cancer

摘要：

　　AIM: To investigate the effect of targeting protein for TPX2-specific siRNA on the raidiosensitivity of Hela cell in Human cervical Cancer.METHODS: The siRNA was introduced by Lipofectamine2000 into Human cervical cancer.RT-PCR and western blot was used to detect the expression of TPX2 mRNA and protein in Hela cells.Hela cells transfected were divided into 4 groups : control grouptransfected groupradiation group and combined treatmemt group.Four groups of cell were exposed to different doses of radiation (0,2,4,6,8Gy).Cell survival ratio was detected by means of CCK8 method.And the detection of the apoptosis rate and cell cycle was completed by FCM.

作者: Li meiling Lu haijun

作者单位: The affiliated hospital of Qingdao university

会议类型: 国内会议

会议名称: 2016年国际肿瘤放射治疗新进展天津论坛暨中国北方肿瘤放射治疗协作组第二届学术会议

会议地点: 天津

会议语种:英文

页码: 254-254

在线出版日期: 2016-06-03（万方平台首次上网日期，不代表论文的发表时间）