Cytological and miRNA expression changes during the vascular cambial transition from the dormant stage to the active stage in Ginkgo biloba L.
Key message:During the cambium activity transition, the cambial zones, storage materials, and several related miRNAs change, providing useful information for understanding the cambial characteristics of trees.Abstract: The vascular cambium of trees is an important lateral meristem.In the temperate zones, vascular cambium activity displays periodicity in perennial plants.However, the mechanisms of the cambial dormancy-activity transition are poorly understood.In this study, we divided the periodicity of cambial activity in Ginkgo biloba L.into dormant, reactive, active, and transformed stages according to the phenological period and changes in the cambial cell layers and sizes.The cytological analysis showed that in the dormant stage, the cambium was composed of 4-6 layers of cells with thick walls, and many starch granules and protein bodies in the cambial and phloem cells.During the reactive phase, the width of the cambial zone and cell layers increased slightly.Additionally, the number of cell layers peaked at to 10-12 in the active stage,the cell wall was the thinnest, and the starch granules and protein bodies in the cells decreased significantly, indicating that storage materials play a key role in cambial cell division and differentiation.We further detected several developmental and tolerance-resistance-related miRNAs.We found that miR159 and miR166 were highly expressed during the reactive stage, while the expression levels of miR164 and miR319 significantly increased during the active stage.Conversely, miR482 and miR2950 were highly expressed during the dormant stage, suggesting that these miRNAs were involved in the regulation of the cambial activity transition.These results provide new insights into the complex mechanisms of cambium during the dormancy to activity transition in gymnosperm trees.
Ginkgo biloba vascular cambium structure miRNA
Jiawen Cui Jianguo Zhao Jingya Zhao Huimin Xu Li Wang Biao Jin
College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants of Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University,Beijing 100083, China
国内会议
江苏邳州
英文
2-16
2017-11-01（万方平台首次上网日期，不代表论文的发表时间）