Multiplex PCR Assay for the Simultaneous Detection of Five Pathogens in Laboratory Animals
Staphylococcus aureus(S.aureus),Pseudomonas aeruginosa(P.aeruginosa),Bordetella bronchiseptica(B.bronchiseptica),Mycoplasma pneumoniae(M.pneumoniae),Pasteurella multocida(P.multocida)are commune pathogens isolated frequently in laboratory animals.Various diagnostic methods have been developed to identify these pathogens.In this report,we developed and evaluated a multiplex PCR for detecting these five pathogens in laboratory animals simultaneously,which is significant in differential diagnosis and screening.The primer concentrations and PCR conditions were optimized.The sensitivity of the multiplex PCR for simultaneous amplification is 10 pg of DNA per target.The specificity of the PCR was demonstrated by detecting pathogen-spiked clinical samples.A total of 182 clinical specimens were tested by the multiplex PCR,and the results were consistent with the results obtained using conventional bacterial isolation methods.In conclusion,the multiplex PCR developed in this report is a specific and sensitive method that can be used for differential diagnosis of five bacterial pathogen infections and screening in laboratory animals.
Staphylococcus aureus Pseudomonas aeruginosa Bordetella bronchiseptica Mycoplasma pneumoniae Pasteurella multocida Multiplex PCR,differential diagnosis
Yan-Bo ZHU Long ZHENG Hong-Yu YOU Wei-Guang LIAN Jian-Min LIU Yan-Ning LI Yu-Rong MENG Dong-Ming ZHANG Jun-Xia WANG
Hebei Key Laboratory for Laboratory Animal Scinences,Department of laboratory Animal Sciences,Hebei Medical University,Hebei,Shijiazhuang 050000,China
国内会议
成都
英文
1-13
2017-09-26（万方平台首次上网日期，不代表论文的发表时间）