Phage display library based antiviral screening:porcine respiratory and reproductive syndrome virus endoribonuclease is a targetfor antiviral screening
Viral endoribonuclease plays a vital role in the physiological and biochemical activities of viral RNA.In our studies on PRRSV,we seek to screen candidate antiviral molecules that target this endoribonuclease.Through 5 rounds of phage-display screening of 12-ammino acid peptides,4 peptides that show high affinity to the viral endoribonuclease were identified.In vitro experiments showed very high antiviral activity of all these 4 peptides.Wherein,the E4 peptide(NIPIKPRPRLMK)showed the highest antiviral activity(IC50 = 39.21μM),yet the lowest cytotoxicity.E4 can directly bind to the endoribonuclease of PRRSV(NSP11)and reduce copy number of viral RNA by 23.68 folds.Through large-scale phage-display screening and following in vitro analysis,we proved that viral endoribonuclease is a potential target of antiviral drugs.
Viral endoribonuclease phage display library porcine reproductive and respiratory syndrome virus antiviral screening antiviral peptide
Zhou Jie Hu Jianhua Shi Zixue Tao Lingyun Gao Cheng
Shanghai Laboratory Animal Research Center,Shanghai,201203,China
国内会议
成都
英文
1-12
2017-09-26（万方平台首次上网日期，不代表论文的发表时间）