Salvianolic acid B alleviates heart failure by inactivating ERK1/2/GATA4 signaling pathway after pressure overload in mice
Background Heart failure(HF)is a dangerous disease that affects millions of patients.Radix Salvia is widely used in Chinese clinics to treat heart diseases.Salvianolic acid B(SalB)is the major active component of Radix Salvia.This study investigated the mechanisms of action and effects of SalB on HF in an experimental mouse model of HF.Methods We created a mouse model of HF by inducing pressure overload with transverse aortic constriction(TAC)surgery for 2 weeks and compared among 4 study groups: SHAM group(n=10),TAC group(n=9),TAC+MET group(metprolol,positive drug treatment,n=9)and TAC+SalB group(SalB,240 mg·kg-1·day-1,n=9).Echocardiography was used to evaluate the dynamic changes in cardiac structure and function in vivo.Plasma brain natriuretic peptide(BNP)concentration was detected by Elisa method.In addition,H9C2 rat cardiomyocytes were cultured and Western blot were implemented to evaluate the phosphorylation of ERK1/2,AKT,and protein expression of GATA4.Results SalB significantly inhibited phosphorylation of Thr202/Tyr204 sites of ERK1/2,but not Ser473 site of AKT,subsequently inhibited protein expression of GATA4 and plasma BNP(< 0.001),and then inhibited HF at 2 weeks after TAC surgery.Conclusions Our data provide a mechanism of inactivating the ERK1/2/GATA4 signaling pathway for SalB inhibition of the TAC-induced heart failure.
Heart failure ERK1/2/GATA4 Salvianolic acid B Pressure overload
Juan Yu Renshan Chen Yafang Tan Jiashin Wu Jianyong Qi Minzhou Zhang Weiwang Gu
Laboratory Animal Center,Southern Medical University,Guangzhou city,Guangdong province,China;Animal AMI Key Laboratory of Chinese Medicine in Guangzhou,Guangdong Province Academy of Chinese Medicine,G Department of Pharmaceutical Sciences,College of Pharmacy,Northeast Ohio Medical University,Rootstow Laboratory Animal Center,Southern Medical University,Guangzhou city,Guangdong province,China
国内会议
成都
英文
1-16
2017-09-26（万方平台首次上网日期，不代表论文的发表时间）