A metabolomics approach based on ultra-high-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry to identify the chemical constituents of the Traditional Chinese Er-Zhi-Pill
Er-Zhi-Pill, which consists of Ligustri lucidi fructus and Ecliptae prostratae herba, is a classical traditional Chinese medicinal formulation widely used as a liver-nourishing and kidney-enriching tonic.To identify the bioactive ingredients of Er-Zhi-Pill and characterize the variation of chemical constituents between co-decoction and mix of individually decocted L.lucidi fructus and E.prostratae herba, a novel metabolomics approach based on ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry in both positive and negative ion modes, was established to comprehensively analyze chemical constituents and probe distinguishable chemical markers.In total, 68 constituents were unambiguously or tentatively identified through alignment of accurate molecular weights within an error margin of 5 ppm, elemental composition and fragmentation characteristics, including 8 constituents which were confirmed by comparing to reference standards.Furthermore,principal component analysis and partial least squares discriminant analysis using Simca-p+ 12.0 software were applied to investigate chemical differences between formulations obtained by co-decoction and mix of individual decoctions.Global chemical differences were found in samples of two different decoction methods, and 16 components, including salidroside, specneuzhenide and wedelolactone, contributed most to the observed differences.This study provides a basic chemical profile for the quality control and further mechanism research of Er-Zhi-Pill.
Er-Zhi-Pill Chemical constituents Metabolomics Traditional Chinese Medicine Formulation
Xunlong Zhong Yanmei Zhong Kangqi Yan Xuerong Xiao Lian Duan Ruolun Wang Laiyou Wang
Department of Pharmacy, the Second Affiliated Hospital Of Guangzhou Medical University, Guangzhou, 5 Central laboratory, Guangdong Pharmaceutical University, Guangzhou, 510006, China Research & Development Department, Guangzhou Baiyunshan Mingxing Pharmaceutical Co., Ltd.Guangzhou, School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, China
国内会议
广州
英文
71-76
2017-12-16（万方平台首次上网日期，不代表论文的发表时间）