Crotonaldehyde induces autophagy-mediated cytotoxicity in human bronchial epithelial cells via PI3K,AMPK and MAPK pathways
Crotonaldehyde is an ubiquitous hazardous pollutant in the environment which can be produced naturally,artificially and endogenously.Acute exposure of crotonaldehyde was reported to induce severe lung injury in humans and experimental animals.However,the exact toxicity mechanisms of crotonaldehyde in organisms have not been fully explored.In the present study,we explored the role autophagy played in the cytotoxicity induced by crotonaldehyde in human bronchial epithelial cells(BEAS-2B),and the pathways that mediated autophagy,including the phosphatidylinositol 3-kinase(PI3K)pathway,the AMP-activated protein kinase(AMPK)pathway and the mitogen-activated protein kinase(MAPK)pathways,were examined and validated.We found that crotonaldehyde induced cytotoxicity and autophagy simultaneously in BEAS-2B cells,and blockage of autophagic flux significantly elevated the viability of BEAS-2B exposed to high concentrations of crotonaldehyde.Crotonaldehyde downregulated the activity of PI3K pathway,and elevated the activities of AMPK and MAPK pathways.Pretreatment of specific agonist or antagonist of these pathways could inhibit autophagy and partly improve the viability.These results suggested that acute exposure of crotonaldehyde induced cell death mediated by autophagy,which might be helpful to elucidate the toxicity mechanisms of crotonaldehyde and contribute to environmental and human health risk assessment.
Crotonaldehyde Autophagy PI3K AMPK MAPK
Limeng Wang Xiang Li Zhihua Yang Xiujie Pan Xingyu Liu Maoxiang Zhu Jianping Xie
Key Laboratory of Separation Science for Analytical Chemistry,Dalian Institute of Chemical Physics,C Key Laboratory of Tobacco Chemistry,Zhengzhou Tobacco Research Institute of CNTC,2 Fengyang Road,Zhe Department of Radiation Toxicology and Oncology,Beijing Institute of Radiation Medicine,27 Taiping R Shanghai Tobacco Group Corporation of CNTC,99 Wansheng South Street,Tongzhou District,Beijing 101121
国内会议
北京
英文
287-296
2017-11-01（万方平台首次上网日期，不代表论文的发表时间）