Simultaneous determination of polycyclic aromatic hydrocarbons and tobacco-specific N-nitrosamines in mainstream cigarette smoke using in-pipette-tip solid-phase extraction and on-line gel permeation chromatography-gas chromatography-tandem mass spectrometry
In this study,a silica/primary secondary amine(SiO2/PSA)was used as an in-pipette-tip solid phase extraction(SPE)sorbent for the simultaneous determination of polycyclic aromatic hydrocarbons(PAHs)and tobacco-specific N-nitrosamines(TSNAs)in mainstream cigarette smoke(MSS).We investigated several parameters including an extraction procedure of total particulate matter,type and amount of sorbent and on-line gel permeation chromatography parameters to obtain optimum conditions for a new strategy to target analytes.Under the optimized conditions,we developed a method for the simultaneous determination of PAHs and TSNAs in MSS by coupling in-pipette-tip SPE procedures to an on-line gel permeation chromatography-gas chromatography–tandem mass spectrometry(on-line GPC-GC–MS2).Our method had limits of detection for target analytes ranging from 0.01 to 0.23 ng/cig.Good linearities were obtained with coefficients of determination(R2)greater than 0.9984 for all target analytes.Good reproducibility was obtained as intra-and inter-day precisions,and the relative standard deviations were less than 11.4 and 13.3%,respectively.The recoveries were in the range of 77.1–108.6%at different concentrations for real samples.Compared to previous standard methods for the determination of PAHs and TSNAs in MSS,our method was highly effective,fast,and had low consumption of organic solvent and a high degree of automation.Finally,our method successfully analyzed PAHs and TSNAs in real samples,and no significant deviations were observed when compared to similar analysis using standard methods.
Silica/primary secondary amine (SiO2/PSA) In-pipette-tip solid phase extraction (SPE) Polycyclic aromatic hydrocarbons (PAHs) Tobacco-specific N-nitrosamines (TSNAs) On-line gel permeation chromatography-gas chromatography/tandem mass spectrometry (on-lin
Yan-Bo Luo Xiao-Jing Chen Hong-Fei Zhang Xing-Yi Jiang Xue Li Xiang-Yu Li Feng-Peng Zhu Yong-Qiang Pang Hong-Wei Hou
China National Tobacco Quality Supervision and Test Center,No.2 Fengyang Street,Zhengzhou High and New Technology Industries Development Zone,Zhengzhou 450001,China
国内会议
北京
英文
16-23
2017-11-01（万方平台首次上网日期，不代表论文的发表时间）