Binding of nicotine to Gastrin I Human studied by fluorescence spectroscopy and nuclear magnetic resonance methods

摘要：

　　The interaction between nicotine and Gastrin I Human(GIH)was studied by fluorescence spectroscopy and nuclear magnetic resonance(NMR)methods.Nicotine had little influence on GIH when the ratio of nicotine and GIH was below 80 ∶ 1.However,with an increasing nicotine concentration,nicotine gradually quenched the intrinsic GIH.NMR showed that nicotine interacted with GIH and the pyrrolidyl ring of nicotine was the major binding function.Hydrogen bonding and van der Waals forces were the major functions in the binding between nicotine and GIH.3D and synchronous fluorescence studies indicated that GIH emission was primarily attributed to the Trp residues.The results obtained from the present work may be useful to understand the role of nicotine in developing smokeless tobacco products.

关键词： Chemical shift Environment change Fluorescence quenching Gastrin I Human Nicotine NMR

作者: LIAO Xiaoxiang FAN Ying TANG Jianguo YUAN Dalin CHEN Yongkuan ZOU Quan GONG Ronggang MOU Dingrong WANG Xiaohui WU Kai DUAN Liyue SUN Qiaomei CHENG Qiang LI Hui

作者单位: Technology Center of China Tobacco Yunnan Industrial Co.,Ltd.,No.118 Hongta Road,Yuxi 653100,Yunnan, Chemical Engineering College,Sichuan University,No.24 South Section 1,Yihuan Road,Chengdu 610065,Chi

会议类型: 国内会议

会议名称: 中国烟草学会2017年学术年会

会议地点: 北京

会议语种:英文

页码: 47-54

在线出版日期: 2017-11-01（万方平台首次上网日期，不代表论文的发表时间）