Isolation and characterization of sixteen novel microsatellite loci in two inbred strains of Chinese hamster(Cricetulus griseus)
Objects The Shanyi inbred A and E strains of Chinese hamster are widely used in biomedical research,but detailed genetic characterization has been lacking.The microsatellite markers developed in this study will be useful for genetic diversity analysis,linkage map construction.Methods we isolated and characterized 16 novel microsatellite loci from a microsatellite-enriched genomic DNA library.These loci were genotyped in 48 animals from the two strains,and the polymorphic information content(PIC)was determined.Results The results showed that in Shanyi A and E populations,14 and 15 loci were found to be polymorphic,respectively,with PIC ranging from 0.1393 to 0.8082 and from 0.1109 to 0.7397.A total of 115 alleles were found across the 16 microsatellite loci in the two populations,and the mean observed heterozygosity(HO)were 0.5191 and 0.4333 for the A and E population,respectively,indicating marked genetic variation within the two populations.Correspondingly,the FST values ranged from 0.002 to 0.9253,with an overall average of 0.1935,indicating significant genetic difference between the two strains.The population differentiation was substantiated by Nei”s genetic distance and full Bayesian analyses computed with STRUCTURE.Conclusions Despite the genetic diversity and differentiation within and between the two inbred populations,population analysis showed that 48 individuals were faithfully allocated into their original populations with high statistical confidence based on these 16 microsatellite loci.Taken together,these results provide detailed genetic characterization of the two Chinese hamster inbred strains and identify novel microsatellite loci as useful genetic markers.
Chinese hamster enriched library microsatellite marker genetic differentiation
GUO-HUA SONG JIA-NING GENG RUO-YU JIA WEN-BIN YUE TIAN-FU LIU SONG-NIAN HU CHEN-ZHAO YANG
Shanxi Key Laboratory of Experimental Animal and Human Disease Animal Models,Shanxi Medical Universi Key Laboratory of Genome Information and Sciences,Beijing Institute of Genomics,Chinese Academy of S Shanxi Key Laboratory of Experimental Animal and Human Disease Animal Models,Shanxi Medical Universi College of Animal Science and Technology,Shanxi Agricultural University,Taigu 030801,China
国内会议
呼和浩特
英文
18-25
2017-09-12（万方平台首次上网日期，不代表论文的发表时间）