Low concentration of caffeine inhibits cell viability,migration and invasion,and induces cell apoptosis of B16F10 melanoma cells
We aimed to explore the effects of low concentration of 1,3,7-trimethylxanthine(caffeine)on melanoma cells,as well as the underlying mechanism.In this work,B16F10 murine melanoma cells were pre-treated with different concentrations(0,50,100,200,400 or 600 μM)of caffeine for 24 h,48 h,or 72 h.The cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay.The migration and invasion assay were assessed by Transwell system.The cell apoptosis were analyzed by Annexin V-Cy5 and propidium iodide(PI)staining.The protein levels of bone morphogenetic protein(BMP)2,BMP4,BMP7,B-cell lymphoma(Bcl-2),and Bax were measured by Western blot.The results showed that caffeine(400 or 600 μM)pretreatment significantly reduced the cell viability at 48 h and 72 h(P < 0.05 or P < 0.01)but not at 24 h.The number of migrated and invaded cells was significantly decreased by caffeine(200,400 or 600 μM)(P < 0.05 or P < 0.01).Moreover,the percentages of apoptotic cells were statistically increased by 200,400 or 600 μM of caffeine(P < 0.05 or P < 0.01).We also observed caffeine(200,400 or 600 μM)significantly down-regulated the levels of BMP2 and BMP4 but not BMP7,and statistically down-regulated the ratio of Bcl-2 to Bax(P < 0.05 or P < 0.01).To conclude,low concentration of caffeine inhibits cell viability,migration and invasion,and induces cell apoptosis of B16F10 melanoma cells.
Caffeine melanoma cell viability migration and invasion cell apoptosis
Ning Wu Bohui Zhu Yiwen Chen Haiping Wang Yi Shi Feng Xu Xue Yu Yanxiang Li Yong Li
Department of Oncology,Shanghai Pudong New Area Gongli Hospital,Shanghai 200135,China
国内会议
合肥
英文
1-9
2017-06-15（万方平台首次上网日期，不代表论文的发表时间）