Effect of Qing”e formula on the in vitro differentiation of bone marrow-derived mesenchymal stem cells from proximal femurs of postmenopausal osteoporotic mice
Background Qing”e formula(QEF),prepared from an ancient Chinese recipe,was previously suggested to regulate bone metabolism and improve bone mineral density in patients with osteoporosis.To study the effects of medicated serum containing QEF on the in vitro differentiation of bone marrow-derived mesenchymal stem cells(BMSCs)isolated from the proximal femurs of postmenopausal osteoporosis(PMOP)mice.Methods Using an established mouse model of PMOP,mononuclear cells were isolated from the bone marrow present in the proximal femurs and cultured.PMOP mice were also randomly divided into four groups: the untreated group(Group A)and the groups treated with respectively low(Group B),medium(Group C),and high(Group D)concentrations of QEF.Serum was isolated from each and used to treat the cultured BMSCs in conjunction with recombinant human bone morphogenetic protein-2(rhBMP-2).Cell morphology,proliferation rates,intracellular alkaline phosphatase(ALP)activity,and transforming growth factor-beta 1(TGF-β1)mRNA expression were evaluated.Results QEF-treated serum,particularly that containing moderate and high concentrations,appears to enhance the rhBMP-2-mediated changes in cell morphology,proliferation,and differentiation(determined via the expression of TGF-β1 mRNA and ALP activity)observed in the BMSCs isolated from PMOP mice.Conclusions QEF may play a role in the prevention and treatment of PMOP by enhancing the activity of rhBMP-2.
osteoporosis bone marrow-derived mesenchymal stem cells Qing”e formula transforming growth factor β
Bo Shuai Lin Shen Rui Zhu Piqi Zhou
Department of Integrated Traditional Chinese and Western Medicine,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,China
国内会议
合肥
英文
1-21
2017-06-10（万方平台首次上网日期，不代表论文的发表时间）