Inhibition of Endoplasmic Reticulum Stress Alleviates Lung Injury Induced by Brain Death
Background Endoplasmic reticulum(ER)stress is associated with a variety of diseases.However,little is known about how ER stress is implicated in BD-induced lung injury.In this study,effect of inhibition of ER stress on BD-induced lung injury was examined.Methods Pulmonary histopathologic change was observed by hematoxylin and eosin(HE).Cells in bronchoalveolar lavage fluid were analyzed by Wright Giemsa staining.Proteins related to the ER stress and apoptosis were examined by western blot and immunohistochemical staining.In addition,the apoptotic cells in lung tissues were observed by using terminal dexynucleotidetransferase-mediated dUTP nick-end labeling assay.Results In BD-induced rats,alveolar wall of the lung tissue was thickened and the infiltration of inflammatory cells was observed.The level of GRP78,p-eIF2α,CHOP and Caspase-12 was highly expressed in BD rats comparing with the control group.Salubrinal,a special inhibitor of eIF2α phosphatase enzymes,reduced the expression of GRP78 and apoptosis markers indirectly.Moreover,NF-κB signaling pathway was activated in BD-induced rats.Then,the level of IL-6 increased significantly.Interestingly,salubrinal could also suppress the expression of IL-6 through inhibiting NF-κB signaling pathway.Conclusions Inhibition of ER stress alleviates BD-induced lung inflammation by regulating NF-κB signaling pathway.
HongweiTang Wenzhi Guo Shengli Cao Bing Yan Hongbo Fang Huapeng Zhang Shuijun Zhang
Department of Hepatobiliary and Pancreatic Surgery,the First Affiliated Hospital of Zhengzhou University;Henan Key Laboratory of Digestive Organ Transplantation
国内会议
北京
英文
1-10
2017-05-19（万方平台首次上网日期，不代表论文的发表时间）