Probucol Improves Erectile Function by Restoring Endothelial Function and Preventing Cavernous Fibrosis in Streptozotocin-Induced Diabetic Rats
OBJECTIVE.To investigate the effects of probucol on erectile function in streptozotocin-induced diabetic rats and explore the underlying mechanisms.METHODS.A total of thirty 12-week-old Sprague-Dawley male rats received a one-time intraperitoneal STZ (60 mg/kg) or vehicle injection after a 12-hour fast.Three days later, the STZ-induced diabetic rats were randomly divided into two groups and were treated with daily gavage feedings of probucol at doses of 0 and 500mg/kg for 12 weeks.A positive control group underwent intraperitoneal injection of saline followed by daily gavage of saline solution.Erectile function was assessed by electrical stimulation of the cavernous nerves with real-time intracavernous pressure measurement.After euthanasia, penile tissue was investigated using immunohistochemistry, Westrn biet, and ELISA to assess the PRMT1/DDAH/ADMA/NOS metabolism pathway.SOD activity and MDA levels were detected by colorimetry.We also evaluated penile histological changes such as smooth muscle contents and Masson”s trichrome stain.RESULTS.Significant recovery of erectile function was observed in the probucol-treated rats than the untreated diabetic rats.The protein expression of DDAH and NOS, cGMP concentrations and SOD activity in cavernous tissue of probucol-treated rats were significantly higher than the untreated diabetic rats.The protein expression of PRMT1, ADMA concentrations and MDA levels in cavernous tissue of probucol-treated rats were significantly lower than the untreated diabetic rats.In addition, probucol treatment markedly augments the ratio of smooth muscle cell/collagen fibril.CONCLUSION.Probucol treatment improves erectile function by restoring endothelial function and preventing cavernous fibrosis in streptozotocin-induced diabetic rats.
ADMA DDAH Probucol Erectile Dysfunction Oxidative Stress PRMT1
Ke-Qin Zhang Dong Chen Ding-Qi Sun Hui Zhang Bo Li Qiang FU
Department of Urology, Shandong Provincial Hospital Affiliated to Shandong University, 324 Jingwuweiqi Road, Jinan 250021, China
国内会议
济南
英文
137-157
2016-09-03（万方平台首次上网日期，不代表论文的发表时间）