Effects of Hyperbaric Oxygen Preconditioning on Caspase-Dependent Apoptosis in Neurons after Spinal Cord Injury in Rats
Objective: Hyperbaric oxygen preconditioning (HBO-PC) can reduce neuronal apoptosis after spinal cord injury (SCI), but the mechanism remains unclear.In this study, we sought to investigate the effects of HBO-PC on motor function and cysteine-aspartic acid-specific protease (caspase)-dependent apoptotic pathways after SCI in rats.Methods: Thirty-six healthy Wistar rats were randomly divided intocontrol (n=12), SCI(n=12) and HBO-PC (n=12) groups.After 10 roundsof HB0-PC, the SCI modelwas inducedin both the HB0-PC and SCI groups.Two weeks later, motor function was determinedusingBasso Beattie Bresnahan (BBB) scoring, spinal MRI testing, spinal cord tissue section analysis and MEP testing.The spinal cord fromT7-9 was also collected to quantify apoptosis-related geneexpression.Results: The spinalcavity and atrophy levels, BBB scores, and MEP resultswere lower in the HB0-PC group than in the SCI group.Theexpression ofapoptosis-related geneswassignificantly increased in the SCI group compared with the CON and HBO-PC groups.Conclusion: HBO-PC can prevent the loss of motor function and nerve conduction due to SCI by modulating the endoplasmic reticulum and mitochondrial apoptosis pathways in neurons after HB0-PC.
Hyperbaric oxygen preconditioning(HB0-PC) spinal cord injury(SCI) apoptosis
Huiqiang Chen Yanyan Zeng Huai Huang Dantong Shen Zhiwei Wang Lei Xue Qibiao Weng Xu Zhang Jing Gu Shuilin Ye Mengyu Yao
Department of Orthopedics, Guangzhou General Hospital of Guangzhou Military Command , Guangzhou 5100 Neurological Rehabilitation, Division Ⅱ.Neurology Specialty Hospital, Guangzhou General Hospital of Nanfang Hospital, Southern Medical Hyperbaric Oxygen Therapy Center, Guangzhou510010, Guangdong Prov Zhujiang Hospital, Southern Medical Hyperbaric Oxygen Therapy Center, Guangzhou 510010, Guangdong Pr
国内会议
广东韶关
英文
98-113
2016-04-21（万方平台首次上网日期，不代表论文的发表时间）