Vascular Endothelial Growth Factor Enhanced the Angiogenesis Response of Human Umbilical Cord-Derived Mesenchymal Stromal Cells in a Rat Model of Radiation Myelopathy
The present study was designed to examine a synergistic role for VEGF and human umbilical cordderived MSCs (UC-MSCs) therapy in a rat model of radiation myelopathy.UC-MSCs and VEGF were injected through the tail vein at 90, 97, 104 and 111 days postirradiation.Behavioral tests were performed, and histological damage was examined.The microcirculation in the spinal cord was assessed using von Willebrand factor immunohistochemical analysis and laser-Doppler flowmetry.The microenvironment in the spinal cord was determined by measuring the pro-inflammatory cytokines interleukin-1β and tumor necrosis factor-α in the serum and the anti-inflammatory cytokines brain-derived neurotrophic factor and glial cell-derived neurotrophic factor in the spinal cord.The UC-MSCs processed with VEGF,including VEGF165-induced UC-MSC (iUC), VEGF and UC-MSCs (VEGF-UC), increased the endothelial cell density and the microvessel density in the white matter and gray matter of the spinal cord, raised the relative magnitude of spinal cord blood flow compared to UC-MSCs treatment alone.Our data provided the first evidence that vascular endothelial growth factor enhanced the angiogenesis response of human umbilical cord-derived mesenchymal stromal cells in a rat model of radiation myelopathy.
Radiation myelopathy Human umbilical cord-derived mesenchymal stromal cells Vascular endothelial growth factor
Hua You Li Wei Jing Zhang Jia-Ning Wang
Affiliated Hospital of the Academy of Military Medical Sciences,Beijing 100071,China Key Laboratory of Birth Defects and Reproductive Health of the National Health and Family Planning C Department of Cardiology,Institute of Clinical Medicine,Hubei University of Medicine,Shiyan 442000,H
国内会议
北京
英文
576-587
2016-01-09（万方平台首次上网日期，不代表论文的发表时间）