miR-144-3p exerts anti-tumor effects in glioblastoma by targeting c-Met

摘要：

　　The study aimed to explore the specific function and mechanism of miR-144-3p in glioblastoma (GBM) cells with different phosphatase and tensin homolog (PTEN) phenotypes.We demonstrated that the miR-144-3p level was significantly down-regulated in glioma compared with the non-neoplastic brain tissues, and decreased with ascending grades.The loss of miR-144-3p effectively predicted the decreased overall survival in glioma patients.Interestingly, the expression of MET was up-regulated and inversely associated with miR144-3p level in glioma tissues.Next, we certified that miR-144-3p specifically bound to MET 3”-untranslated region (3”UTR)and inhibited its expression.miR-144-3p potently repressed GBM cell proliferation and invasion via suppressing MET in vitro and in vivo.In addition, our results showed no difference in malignancy inhibition induced by miR-144-3p in GBM cells with different PTEN phenotypes.miR-144-3p inhibited several survival signaling pathways by targeting MET independent of PTEN status in GBM cells.Overexpression of miR-144-3p inhibited survival capability and increased apoptosis, resulting in enhancement of radiation and temozolomide sensitivity.Our data provide new insights into the potential application of miR-144-3p in GBM therapy by targeting MET and then inhibiting the downstream signaling.

关键词： c-Met glioblastoma irradiation miR-144-3p temozolomide

作者: Fengming Lan Huiming Yu Man Hu Tingyi Xia Xiao Yue

作者单位: Department of Radiation Oncology,Tianjin Hospital,Tianjin,China Key laboratory of Carcinogenesis and Translational Research (Ministry of Education),Department of Ra Department of Radiation Oncology,Shandong Cancer Hospital,Shandong Academy of Medical Sciences,Jinan Department of Radiation Oncology,PLA Airforce General Hospital,Beijing,China Tianjin Huanhu Hospital,Tianjin,China

会议类型: 国内会议

会议名称: 2015年北京放射肿瘤年会

会议地点: 北京

会议语种:英文

页码: 677-689

在线出版日期: 2016-01-09（万方平台首次上网日期，不代表论文的发表时间）