Anti-TWEAK monoclonal antibodies reduce vascular damage and leukocytes infiltration in a mouse model of cutaneous Arthus reaction
Summary Background.Tumor necrosis factor-like weak inducer of apoptosis (TWEAK), as a pro-inflammatory cytokine, is closely associated with the pathogenesis of various types of cutaneous vasculitis (CV).Aim.To investigate the therapeutic effects of anti-TWEAK monoclonal antibody (mAb) in a mouse model of cutaneous reverse passive Arthus (RPA) reaction.Methods.Cutaneous RPA reaction was induced in BALB/c mice by injection intradermally with antiovalbumin IgG into the left ear followed immediately by i.v.injection of chicken ovalbumin.After treatment, the hemorrhagic lesions in mice skin were observed by a semiquantitative score.The amount of extravasated FITC-labeled bovine serum albumin (BSA) in the ears was detected spectrophotometrically.The expression of myeloperoxidase (MPO) was observed by immunohistochemical staining.The mRNA expression of tumor necrosis factor-α (TNF-oα) and interleukin-6 (IL-6) in lesional skin was detected by real-time quantitative PCR.Results.Our results indicated that anti-TWEAK mAb significantly attenuated the clinical and histopathologic changes in immune complex (IC) induced mice.Anti-TWEAK mAb also reduced semiquantitative score for hemorrhage, the extravasation of FITC-labeled BSA and the activities of MPO.Finally, by real-time quantitative PCR, we showed that anti-TWEAK mAb significantly inhibited the mRNA expression of TNF-α and IL-6 in lesional skin from IC-induced mice.Conclusion.These data suggest that anti-TWEAK mAb can block vascular damage and leukocytes infiltration in IC-induced mice.It might be a candidate immunotherapeutic medicine for the suppression of IC-induced CV.
Tao Chen Zai-pei Guo Li-xin Fu Na Cao Sha Qin
Department of Dermatovenereology, Chengdu Second People”s Hospital, Chengdu,610041, Sichuan, China Department of Dermatovenereology, West China Hospital of Sichuan University,Chengdu, 610041, Sichuan
国内会议
第十届四川·重庆皮肤性病学术会议暨四川省医学会第十三次皮肤性病学术会议
成都
英文
81-89
2016-01-08（万方平台首次上网日期，不代表论文的发表时间）