Analysis of differentially expressed proteins during post-harvest maturation of white button mushroom Agaricus bisporus
In order to identify the protein expression changes that occur during the post-harvest maturation of Agaricus bisporus fruitbodies, the technique of iTRAQ-MS/MS (i.e.isobaric tags for relative and absolute quantification coupled two-dimensional liquid chromatography tandem mass spectrometry) was used to analyze the proteoms of A.bisporus strain As2796 fruitbodies at 0h, 6h, 12h and 48h after harvesting.As a result, 1063 proteins, included a total of 5878 unique peptides, were identified.Among them, 1012 proteins provided quantitative information.Compared with the proteins at 0h post harvest, samples collected at 6h, 12h, 48h after harvesting produced 102, 106, and 160 different proteins respectively, accounting for 10.1%, 10.5% and 15.8% of the proteins identified.The differentially expressed proteins among different post-harvest stages, as well as all of the identified proteins, were analyzed via bioinformatics.Furthermore, the related genes of 7 proteins either up or down expressed gradually at different post-harvest stages were analyzed by real-time PCR, and 3 of them, mismatched base pair and cruciform DNA recognition protein, hydrophobin-B and protein transporter, were confirmed to have a similar expression trend as the corresponding proteins.
Agaricus bisporus iTRAQ-MS/MS Edible fungus Proteomics Protein identification Real-time PCR
Mei-Yuan Chen Jian-Hua Liao Hong-Rong Li Zhi-Xin Cai Zhong-Jie Guo Mark P.Wach Ze-Sheng Wang
Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou 350014, PR China Sylvan Research, West Hills Industrial Park, Bldg 2.Kittanning,PA 16201, USA
国内会议
福建省科协第十六届学术年会分会暨2016年福建省食用菌学会学术年会
福州
英文
34-43
2016-08-20（万方平台首次上网日期，不代表论文的发表时间）