Beyond gap junction channel function: the expression of Cx43 contributes to aldosterone-induced mesangial cell proliferation via the ERK1/2 and PKC pathways
Aims: This study aimed to explore the precise mechanism and signaling pathways of mesangial cell (MC) proliferation from a new point of view considering Connexin 43 (Cx43).Methods: MC proliferation was measured by the incorporation of 3H-thymidine (3H-TdR).Cx43 was over-expressed in MC cells using lipofectamine 2000, and the expression level was tested with reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analyses.The gap junction channel function was explored by Lucifer Yellow scrape loading and dye transfer (SLDT), and the intracellular calcium concentrations (”Ca2+”i) were characterized by confocal microscopy on cells loaded with Fura-3/AM.
Connexin 43 Gap junction mesangial cells proliferation
韩瑛
淄博市妇幼保健院儿科 255029
国内会议
济南
英文
207-209
2016-05-01（万方平台首次上网日期，不代表论文的发表时间）