Molecular Cloning and Functional Analysis of an Ethylene Receptor Gene from Sugarcane(Saccharum spp.)by Hormone and Environmental Stresses
Ethylene receptor(ethylene response sensor,ERS)is the primary component involving in the ethylene biosynthesis and ethylene signal transduction pathway.In the present study,a GZ-ERS gene encoding ERS was cloned from a sugarcane cv.YL17(Saccharum spp.)using RT-PCR and ligation-mediated PCR with primers designed based on conserved amino acid sequences of ERS from rice and corn.The GZ-ERS gene was 2,243 base pairs in length and coded for a polypeptide of 633 amino acid residues.The amino acid sequence of GZ-ERS encoded protein was 97,96 and 91%identical to OS-ERS(AAX95525)of rice,ZmERS25(AAR25567)of corn,and Sc-ERS(ACF60981)of a sugarcane cv.ROC22,respectively.Genomic Southern analysis using a GZ-ERS cDNA insert as the probe indicated the presence of a single copy of GZ-ERS gene per haploid sugarcane genome.Real-time qPCR analysis showed that GZ-ERS was widely expressed in the vegetative tissues of sugarcane,and its expression in maturing internodes and leaf tissues was higher than in immature tissues.Furthermore,the expression level of GZ-ERS was the highest in maturing leaves,but very low in immature internodes.The GZ-ERS expression level in maturing leaves was enhanced by ethephon treatment,but was reduced under low temperature,drought,and dark conditions.The results from this study may help understand the regulation of gene expression in sugarcane and its responses to plant growth regulator ethephon and environmental stresses.As the sugarcane ERS family has multiple members,other members need to be isolated and characterized in future studies.
Ethylene receptors Cloning Sugarcane Gene expression Real-time qPCR
Ai-Qin Wang Xing-Zhi Ye Jing-Li Huang Jun-Qi Niu Ming Liu Yong-Bao Pan Li-Tao Yang Yang-Rui Li
Key Laboratory of Sugarcane Biotechnology and Genetic Improvement(Guangxi),Ministry of Agriculture,N State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresource,College of Agr United States Department of Agriculture-Agricultural Research Service,Mid-South Area,Sugarcane Resea
国内会议
广西来宾
英文
1,23-30
2015-09-06（万方平台首次上网日期，不代表论文的发表时间）