MicroRNA-340targets NF-κB1 to inhibit cell proliferation, migration and invasion in hepatocellular carcinoma cell line
Aberrant expression of microRNA-340 (miR-340) has been frequently reported in some cancers excluding hepatocellular carcinoma (HCC).The role and its molecular mechanism of miR-340 in HCC have not been reported.In this study, we explored the effects of miR-340 overexpression on apoptosis, invasion and epithelial-mesenchymal transition (EMT) in HCC cells.The mRNA level of miR-340 in HCC cell lines was determined by real-time PCR.The miR-340 mimic was transiently transfected into HCC cells using LipofectamineTM 2000 reagent.Subsequently, the Brdu-ELISA results showed that introduction of miR-340 inhibited cell proliferation.Our data also demonstrated that miR-340 mimic arrested cell cycle progression and promoted apoptosis of HepG2 and MHCC-97L cells.In addition, miR-340 overexpression could also inhibit invasion and EMT of HCC cells.qRT-PCR were used to determined the expressions of matrix metalloproteinase-2 and-9 (MMP-2 and-9) in HCC cells.Next, we found that NF-κB1 expression was evidently reduced by up-regulation of miR-340.Bioinformatics analysis predicted that the NF-κB1 was a potential target gene of miR-340.Luciferase reporter assay further confirmed that miR-340 could directly target the 3”UTR of NF-κB1.In conclusion, miR-340 plays an important role in inhibiting proliferation, migration, EMT and inducing apoptosis by down-regulation of NF-κB1, suggesting the possibility of miR-340-based therapeutics in HCC.
MicroRNA-340 Hepatocellular carcinoma Proliferation Invasion NF-κB1
Chengliang Yuan Chaohong Liu Ning Zou
Department of Liver Diseases, Deyang City People”s Hospital, Deyang, 618000, People”s Republic of China
国内会议
成都
英文
375-387
2016-10-20（万方平台首次上网日期，不代表论文的发表时间）