Corn seeds as bioreactors for the production of phytase in the feed industry
Corn seed is a major ingredient of animal feed worldwide.However, it contains phytate, a major phosphate storage form that is unavailable to monogastric animals like pigs and poultry.We report a transgenic corn with bioavailable phosphate, achieved by seed-specific overexpression of Aspergillus niger phytase,an enzyme catalyzing the release of phosphate from phytate.We obtained maximal phytase activity of 125 FTU/g kernels, 1000-fold above that of the wild type, with 1000 g of kernels containing up to 67 times the feed industry requirement.Enzymatic characterization ofZea mays recombinant phytase (ZmrPhy) showed it to be equivalent to yeast (Pichia pastoris) recombinant phytase (PprPhy), a commercially available phytase product.An animal feeding trial demonstrated that ZmrPhy had similar nutritional effects on broiler chickens to PprPhy in terms of reducing inorganic phosphorus addition to feed and phosphate excretion in animal manure.These results suggest that transgenic phytase corn can be used directly in the feed industry.Experiments were conducted to assess the food safety of the corn;the results demonstrated no difference versus regular corn.This is the first genetically modified corn officially issued with a biosafety certificate in China and has great potential in the animal feed industry.
Phytase Corn endosperm Phosphate phytate Animal feed
Rumei Chen Chunyi Zhang Bin Yao Guangxing Xue Wenzhu Yang Xiaojin Zhou Junmin Zhang Cheng Sun Ping Chen Yunliu Fan
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;Na Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Beijing 100081, China State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultur
国内会议
北京
英文
189-195
2016-03-29（万方平台首次上网日期，不代表论文的发表时间）