GmTMT2a from soybean elevates the α-tocopherol content in corn and Arabidopsis
Tocochromanol, or vitamin E, plays a crucial role in human and animal nutrition and is synthesized only by photosynthetic organisms.γ-Tocopherol methyltransferase (γ-TMT), one of the key enzymes in the tocopherol biosynthetic pathway in plants, converts γ, δ-tocopherols into α-, β-tocopherols.Tocopherol content was investigated in 15 soybean cultivars and GmTMT2 was isolated from five varieties based on tocopherol content.GmTMT2a was expressed in E.coli and the purified protein effectively converted γ-tocopherol into α-tocopherol in vitro.Overexpression of GmTMT2a enhanced α-tocopherol content 4-6-fold in transgenic Arabidopsis, and α-tocopherol content increased 3-4.5-fold in transgenic maize seed, which correlated with the accumulation of GmTMT2a.Transgenic corn that is α-tocopherol-rich may be beneficial for animal health and growth.
Glycine Max Maize Vitamin E Tocopherol γ-Tocopherol methyltransferase (γ-TMT) α-Tocopherol GmTMT2a
Lan Zhang Yanzhong Luo Yongxing Zhu Liang Zhang Wei Zhang Rumei Chen Miaoyun Xu Yunliu Fan Lei Wang
Biotechnology Research Institute, National Key Facility of Crop Gene Resources and Genetic Improveme Agricultural Bio-Technology Center, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 7
国内会议
北京
英文
196-203
2016-03-29（万方平台首次上网日期，不代表论文的发表时间）