Overexpression of an Acidic Endo-β-1,3-1,4-glucanase in Transgenic Maize Seed for Direct Utilization in Animal Feed
Background: Incorporation of exogenous glucanase into animal feed is common practice to remove glucan, one of the antinutritional factors, for efficient nutrition absorption.The acidic endo-β-1,3-1,4-glucanase (Bgl7A) from Bispora sp.MEY-1 has excellent properties and represents a potential enzyme supplement to animal feed.Methodology/Principal Findings: Here we successfully developed a transgenic maize producing a high level of Bgl7AM (codon modified Bgl7A) by constructing a recombinant vector driven by the embryo-specific promoter ZM-leg1A.Southern and Western blot analysis indicated the stable integration and specific expression of the transgene in maize seeds over four generations.The β-glucanase activity of the transgenic maize seeds reached up to 779,800 U/kg, about 236-fold higher than that of non-transgenic maize.The β-glucanase derived from the transgenic maize seeds had an optimal pH of 4.0 and was stable at pH 1.0-8.0, which is in agreement with the normal environment of digestive tract.Conclusion/Significance: Our study offers a transgenic maize line that could be directly used in animal feed without any glucanase production, purification and supplementation, consequently simplifying the feed enzyme processing procedure.
Yuhong Zhang Xiaolu Xu Xiaojin Zhou Rumei Chen Peilong Yang Qingchang Meng Kun Meng Huiying Luo Jianhua Yuan Bin Yao Wei Zhang
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P.R.China Key Laboratory for Feed Biotechnology of the Ministry of Agriculture,Feed Research Institute, Chines Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, P.R.China
国内会议
北京
英文
227-234
2016-03-29（万方平台首次上网日期，不代表论文的发表时间）