一种老药新用的方法学探究
Drug repurposing is much more efficient and economic than traditionaldrug development because the repositioned drug with known safety reducesthe risk of failure for reasons of adverse toxicology are reduced. HighlypathogenicaVlan influenza A virus could lead to human serious respiratoryinjuriesand consequently acute lung injury (ALI) oracute respiratory dis-tress syndrome (ARDS). The limited therapeutic strategies make the mortality rate of humans infected with HSNla.vian influenza virus remain high.1n our study， ahigh efficiency ”omic” method was established in looking forrepurposing drugs in AL1 induced by avian influenza. We obtained the transcriptome data of the cells infected with avian influenza A HSNl virus by RNA-sequencing technique. After RNAseq dataquality control and genom-ic mapping, we screened the differentially expression genes with HSNl virusinfection. Metacore software was used for genes functional enrichment cluster analysisand filtered the enriched signaling pathways including differentially expressed genes. Basing on drugtarget database DrugBank and Therapeutic Target Database (TTD), we selected the candidate drugs for treat-ment ofAL1 induced by HSNl virus. Our study showed several clinical drugswere effective in treating avian influenza A HSNl virus infection both invitro and in vivo. A new highefficiency repurposing drugs method wasestablished for disease potential treatment searching.
病毒感染 临床药理 抗病毒活性
蒋澄宇
北京协和医学院基础学院
国内会议
北京
中文
8-10
2017-06-17（万方平台首次上网日期，不代表论文的发表时间）