High-Frequency Electroacupuncture Rescues Hippocampal Synapse Ultrastructure And Ameliorates Cognitive Impairment of Rats with Aβ1-42-Induced Alzheimer”s Disease via Inhabiting GSK-3β
Acupuncture has been shown to ameliorate the cognitive impairment of Alzheimer”s disease(AD).However,the underlying mechanism remains to be validated.This study aimed to investigate whether electroacupuncture(EA)could ameliorate cognitive deficit and modify the synapse ultrastructure in Aβ1-42-induced rat model through inhabiting GSK-3β.The AD model was established by the intracerebroventricular administrations of Aβ1-42 into the hippocampus.All rats were evaluated for their capabilities of spatial navigation and memorization by Morris water maze.Synapse ultrastructure was analyzed by electron microscopy.Western blotting tested protein levels of total GSK-3β,pSer9-GSK-3β,pTyr216-GSK-3β,APP and Aβ1-40.After 14 days of treatment,EA significantly improved behavioral deficits compared with the Aβ1–42-injected group.Electroacupuncture also significantly increased the synaptic curvatures,decreased the width of synaptic cleft,thickened the postsynaptic density,and downregulated the expression of GSK-3β,APP and Aβ1-40.pSer9-GSK-3β was markedly decreased,while pTyr216-GSK-3β was increased.And high-frequency EA has better therapeutic effects compared to low and medium-frequency EA.In conclusion,High-frequency EA exerts a protective effect against Aβ1–42-induced learning and memory deficits and synapse ultrastructure impairment via inhabiting GSK-3β activity.
Chao-Chao Yu Ying Wang Feng Shen Li-Hong Kong Ya-Wen Wang Hua Zhou Lei Tang
Hubei University of Chinese Medicine,Wuhan,Hubei 430061,China Department of Acupuncture and Moxibustion,College of Acupuncture and Orthopedics,Hubei University of Department of Rehabilitation,Wuhan Central Hospital Affiliated to Tongji Medical College,Huazhong Un
国内会议
北京
英文
1-14
2017-12-01（万方平台首次上网日期，不代表论文的发表时间）