An innovative method for enrichment of high-polarity bioactive molecules present at low concentrations in complex matrices
Ginsenoside Rg1 is a valuable bioactive molecule,but its high polarity and low concentration in complex mixtures makes it a challenge to separate Rg1 from other saponins with similar structures,resulting in low extraction efficiency.The successful development of effective Rg1 molecularly imprinted polymers(MIPs)that exhibit high selectivity and adsorbability may offer an improved method for the enrichment of active compounds.In this work,MIPs were prepared with two different methods,precipitation polymerization or surface imprinted polymerization.Comparison of the adsorption abilities showed higher adsorbability for the SMIPs prepared by surface imprinted polymerization,46.80 mg/g,compared to the 27.74 mg/g observed for the MIPs prepared by precipitation polymerization.Therefore,for higher adsorbability of the highly polar Rg1,surface imprinted polymerization is a superior technique to make Rg1 MIPs.The prepared surface molecularly imprinted polymers(SMIPs)were tested as a solid phase extraction column to directionally enrich Rg1 and its analogues from ginseng tea and total ginseng extracts.The column with SMIPs showed higher enrichment efficiency and better selectivity than a C18 solid phase extraction column.24 h treatment with eluates from SMIPs-SPE column decreased the apoptotic and necrotic cortical neurons.As a result the Bcl-2/Bax ratio was enhanced significantly compared to the OGD/R group.Cells were treated with the indicated samples for 24 h.The levels of gene expression were assessed as the ratio of Bcl-2/Bax percentage.Overall,a new,innovative method was developed to efficiently enrich high-polarity bioactive molecules present at low concentrations from in complex matrices.
Surface molecularly imprinted polymers High-polarity bioactive molecule Ginsenoside Rg1 Ginsenoside Rb1 Ginsenoside Re
Qing-Shan Liu Li-na Yi Huo-qiang Huang Wen-Bin Zhou Yu-Long Gu Tian-Hua Yan Rui Liu
Key Lab of Ministry of Education,National Research Center for Chinese Minority Medicine,Minzu Univer Key Lab of Ministry of Education,National Research Center for Chinese Minority Medicine,Minzu Univer College of Pharmacy,China Pharmaceutical University,210009,Nanjing,China Institute of Materia Medica,Peking Union Medical College & Tsinghua University,Beijing 100050,China
国内会议
北京
英文
1-5
2016-12-01（万方平台首次上网日期，不代表论文的发表时间）