Genome-wide investigation of the NAC transcription factor familyin melon(Cucumis melo L.)and their expression analysis under salt stress
(0) NAC transcription factor proteins play important roles in many biological processes,including plant development and stress responses.To date,few full-length melon NAC proteins have been identified.In this study,82 melon NAC(CmNAC) genes were identified in the Cucumis melo L.genome.By interrogating our cDNA libraries and transcriptome data from melon under salt stress,and comparison of their phylogenetic relationship with Arabidopsis NAC salt stress-related genes,we putatively identified that the fourth clade of CmNAC genes were involved in the salt stress response,especially the second clade of the group IV of the phylogenetic tree.Expression analysis confirmed that eleven of the twelve CmNAC genes from the group IV were induced in melon seedling roots by salt stress;the other gene was down regulated by salt stress.The expression of CmNAC14 continually increased in 12h under salt stress,and was selected for transformation into Arabidopsis for functional verification.Overexpression of CmNAC14 increased the sensitivity of transgenic Arabidopsis lines to salt stress,which were simultaneously demonstrated by reduced expression of abiotic stress-response genes and variation in several physiological indices.This study increases our knowledge and may enable further characterization of the roles of CmNAC family in the response to salt stress.
Shiwei Wei Liwei Gao Yidong Zhang Furong Zhang Xiao Yang Danfeng Huang
Department of Plant Science,School of Agriculture and Biology,Shanghai Jiao TongUniversity,800 Dongchuan Road,Shanghai 200240,China;Key Laboratory of Urban Agriculture(South),Ministry of Agriculture,Shanghai,China
国内会议
上海
英文
3-3
2016-10-29（万方平台首次上网日期，不代表论文的发表时间）