Two-mAb cocktail protects macaques against the Makona variant of Ebola virus

摘要：

　　The 2014-2015 Ebola virus(EBOV)outbreak in West Africa highlighted the urgent need for specific therapeutic interventions for infected patients.The human-mouse chimeric monoclonal antibody(mAb)cocktail ZMapp,previously shown to be efficacious in EBOV(variant Kikwit)lethally infected nonhuman primates(NHPs)when administration was initiated up to 5 days,was used in some patients during the outbreak.We show that a twoantibody cocktail,MIL77E,is fully protective in NHPs when administered at 50 mg/kg 3 days after challenge with a lethal dose of EBOV variant Makona,the virus responsible for the ongoing 2014-2015 outbreak,whereas a similar formulation of ZMapp protected two of three NHPs.The chimeric MIL77E mAb cocktail is produced in engineered Chinese hamster ovary cells and is based on mAbs c13C6 and C2G4 from ZMapp.The use of only two antibodies in MIL77E opens the door to a pan-ebolavirus cocktail.

作者: Xiangguo Qiu Jonathan Audet Ming Lv Shihua He Gary Wong Haiyan Wei Longlong Luo Lisa Fernando Andrea Kroeker Hugues Fausther Bovendo Alexander Bello Feng Li Pei Ye Michael Jacobs Giuseppe Ippolito Erica Ollmann Saphire Shengli Bi Beifen Shen George F.Gao Larry Zeitlin Jiannan Feng Boyan Zhang Gary P.Kobinger

作者单位: Department of Medical Microbiology,University of Manitoba,Winnipeg,Manitoba R3E 0J9,Canada;National Department of Medical Microbiology,University of Manitoba,Winnipeg,Manitoba R3E 0J9,Canada Laboratory of Immunology,Institute of Basic Medical Sciences,Taiping Road ＃27,Beijing 100850,China National Microbiology Laboratory,Public Health Agency of Canada,Winnipeg,Manitoba R3E 3R2,Canada National Microbiology Laboratory,Public Health Agency of Canada,Winnipeg,Manitoba R3E 3R2,Canada;Ins Beijing Mab-works Biotech Co.Ltd,No.99,Kechuang 14th Street,Economic-Technological Development Area, Rcyal Free London NH5(National Health Service)Foundation Trust,Pond Street,London NW3 2QG,UK National Institute for Infectious Diseases ”Lazzaro Spallanzani” Rome 00149,Italy Department of Immunology and Microbial Science,The Scripps Research Institute,La Jolla,CA 92037,USA; National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and P CAS Key Laboratory of Pathogenic Microbiology and Immunology,Institute of Microbiology,Cninese Acade Department of Medical Microbiology,University of Manitoba,Winnipeg,Manitoba R3E 0J9,Canada;National

会议类型: 国内会议

会议名称: 第八届国际分子模拟与信息技术应用学术会议

会议地点: 大连

会议语种:英文

页码: 1026-1036

在线出版日期: 2016-09-24（万方平台首次上网日期，不代表论文的发表时间）