TALENs and CRISPR/Cas9 system mediated bi-allelic and multiplegene modifications in pigs at one step
Pigs are important for agriculture and study of human diseases.Transcription activator-like effector nucleases(TALENs)and clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated(Cas)9 system(CRISPR/Cas9)show enormous potential for gene engineering in various species,including pigs.Here,we used TALENs and CRISPR/Cas9 system to target single genes(GGTA 1,Parkin,DJ-1)and triple genes(Parkin/DJ-1/P I N K 1)in the porcine genome.The targeting efficiency of TALENs was 2.5%-22%,while the CRISPR/Cas9 system reached an efficiency of up to 100%.DJ-1 knockout(KO)piglets were generated by somatic cell nuclear transfer from targeted fibroblasts and Parkin/DJ-1/P I N K 1 triple targeted pigs were generated by direct embryo injection.These pigs would provide valuable information for studying Parkinson”s disease in human.
TALENs CRISPR/Cas9 pig gene targeting Parkinson”s disease
Yao jing Cao chunwei Huang jiaojiao Wang xianlong Hai tang Zheng qiantao Wang xiao Zhang hongyong Qin guosong Cheng jinbo Yuan zengqiang Wang hongmei Zhou qi Zhao jianguo
State Key laboratory of Reproductive Biology;Beijing Farm Animal Research Center,Institute of Zoolog State Key Laboratory ofBrain and Cognitive Sciences,Institute of Biophysics,Chinese Academy of Scien
国内会议
重庆
英文
51-57
2016-05-25（万方平台首次上网日期，不代表论文的发表时间）