Transcriptional analysis of porcine circovirus-like virus P1

摘要：

　　Background Recently identified porcine circovirus-like virus P1 has the smallest DNA viral genome.In this study,we identified the viral genes and their corresponding mRNA transcripts.Results The RNAs of P1,synthesized in porcine kidney cells,were examined with northern blotting and PCR analyses.Eight virus-specific RNAs were detected.Four mRNAs(open reading frames(ORFs)1,2,4,and 5)are encoded by the viral(-)strand and four(ORFs 3,6,7,and 8)are encoded by the viral(+)strand.All proteins encoded by the ORFs of the P1 virus are less than 50 amino acids in length,except that encoded by ORF1(113 amino acids).Conclusions We show a very complex viral transcription pattern in P1-infected cells.

关键词： Porcine circovirus like virus P1 Transcriptional analysis Northern blotting RACE

作者: Li-bin Wen Feng-zhi Wang Kong-wang He Bin Li Xiao-min Wang Rong-li Guo Jian-ping Xie

作者单位: Jiangsu Academy of Agricultural Sciences · Key Laboratory of Veterinary Biological Engineering and Technology,Ministry of Agriculture · National Center for Engineering Research of Veterinary Bio-products,Institute of Veterinary Medicine,Nanjing 210014,China

会议类型: 国内会议

会议名称: 第七届中国畜牧科技论坛

会议地点: 重庆

会议语种:英文

页码: 500-512

在线出版日期: 2016-05-25（万方平台首次上网日期，不代表论文的发表时间）