Effect of selenium supplementation on apoptosis and cell cycle blockage of renal cells in broilers fed a diet containing aflatoxin B1
The aim of the study was to investigate the competency of selenium(Se)in counteracting the adverse effects of aflatoxin B1(AFB1)on apoptosis,cell cycle and proliferation of nephritic cells.240,one-day-old healthy male avian broilers were randomly divided into four groups and fed with basal diet(control group),0.3 mg/kg AFB1 diet(AFB1 group),0.4 mg/kg Se diet(+Se group)and 0.3 mg/kg AFB1+0.4 mg/kg Se diet(AFB1+Se group),respectively.Compare to the control group,the number of apoptotic renal cells,expressions of Bax and Caspase-3 mRNA were significantly increased,while the expression of Bcl-2 was significantly decreased in AFB1 and+Se groups(P<0.01).A significant decreased proliferating cell nuclear antigen(PCNA)expression and arrested G0/G1 phases of the cell cycle were also seen in the AFB1 and+Se groups when compared with those of the control group.Moreover,these parameters were restored to control group levels in the AFB1+Se group.These results suggested that sodium selenite supplied in the diet,could effectively inhibit AFB1-induced apoptosis and cell cycle blockage in renal cells of broiler.
Aflatoxin B1 Selenium Renal cell Apoptosis Cell Cycle Broiler
Zhengqiang Yu Fengyuan Wang Na Liang Chuhan Wang Xi Peng Jing Fang Hengmin Cui Muhammad Jameel Weimin Lai
Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province,College of Veterinary Medicine,Sichuan Agricultural University,Ya” an,Sichuan 625014,PR China
国内会议
重庆
英文
867-884
2016-05-25（万方平台首次上网日期，不代表论文的发表时间）