Prediction of Mycoplasma hyopneumoniae proteins” subcellular localization based on 5 genome-sequenced strains
Background: Acquiring the subcellular localization of proteins in Mycoplasma hyopneumoniae(M.hyopneumoniae),the etiological agent of porcine enzootic pneumonia,is helpful for understanding the pathogenic mechanism and searching for candidate protective antigens for a more effective vaccine.Although some proteins” localizations have been identified experimentally or annotated in genomic data in previous studies,most of the proteins” localizations are unknown or unclear.Results: We predicted and manually curated the subcellular localization of proteins in M.hyopneumoniae strain 232 by following a conducting workflow and some criteria that incorporated computational predictions of signal peptides,secretion systems,transmembrane domains,protein domain content,experimental data,etc.Meanwhile,the conservation of proteins was analyzed by using all five genome-sequenced and annotated M.hyopneumoniae strains.With comprehensive prediction,data incorporation and manual curation,we concluded that M.hyopneumoniae contains 650 conserved proteins,including 390 cytoplasmic proteins,168 inner membrane proteins,23 periplasmic proteins,136 outer membrane proteins and 3 extracellular proteins(some proteins have multiple localizations).Computational predictions,conducted at the M.hyopneumoniae genome scale,provide less costly localization information,which might not be readily derived from functional annotations but solely from sequence similarity.Conclusions: The localization prediction of all of the M.hyopneumoniae proteins is not simply a matter of developing bioinformatics information.It will also be useful for localization identification experiments,pathogenic mechanism studies,more effective antigenic candidate protein discoveries,and new vaccine development.
Mycoplasma hyopneumoniae subcellular localization prediction manually curated outer membrane extracellular
Honglei Ding Qinhuan Fu Xianzhong Wang Haoju Wang
Laboratory of Veterinary Lemology,College of Animal Science and Technology,Southwest University,Beibei,Chongqing,China 400715
国内会议
重庆
英文
986-1002
2016-05-25（万方平台首次上网日期，不代表论文的发表时间）