Morphogenesis and Proliferative Rule of Porcine Transmissible Gastroenteritis Virus in SIEC
In order to get a better understanding of the replication,proliferation and infection characteristics of porcine transmissible gastroenteritis virus(TGEV)in immortalized swine intestinal epithelial cells(SIEC),this study established the cell model of SIEC infected with TGEV Chongqing(CQ)strain,and the morphogenesis and proliferative rule of TGEV in SIEC were investigated in cytology by transmission electron microscopy(TEM)and indirect immunofluorescence assay(IFA),laser scanning confocal microscope technology(LSCM)and real-time fluorescence quantitative PCR(FQ-PCR).Observations of ultrathin sections under TEM indicated that enveloped viral particles were roughly spherical with diameters of between 80 and 120 nm,and those virions entered SIEC by membrane fusion and the mature viruses in the vacuoles were transported to the cell membrane before release out.The experimental results also found that during the time period 0-12h after TGEV attacked SIEC,intracellular content of viral RNA was not significantly difference,and it showed a logarithmic growth in the 12-36h,thereafter gradually decreased.Moreover,the extracellular RNA content began to rise at 24h after inoculation and reduced gradually at approximately 48h.This study provides a theory foundation for further study on the infection characteristics of TGEV in target cells.
Transmissible gastroenteritis virus Morphogenesis Swine intestinal epithelial cell
Xianjin Dai Cuifang Ye Yuntian Li Li Wang Yang Hu Zhenhui Song
Department of Veterinary Medicine,Southwest University,Rongchang Campus,Chongqing 402460,P.R.China
国内会议
重庆
英文
1059-1072
2016-05-25（万方平台首次上网日期，不代表论文的发表时间）