Molecular characterization and differential expression of multiple goose dopamine D2 receptors
Dopamine D2 receptor(DRD2)gene,a member of the dopamine receptors gene family,has been studied as a candidate gene for broodiness due to its special effects on avian prolactin secretion.Here,the genomic DNA and cDNA sequences of goose(Anser cygnoides)DRD2 gene were cloned and characterized for the first time.The goose DRD2 cDNA is 1353 bp in length and encodes a protein of 450 amino acids.The length of goose DRD2 genomic DNA is 8350 bp,including seven exons and six introns.We identified four goose DRD2 variants,which were generated due to alternative splicing.Bioinformatics analysis indicates that all the deduced DRD2 amino acid sequences contain seven putative transmembrane domains and four potential N-glycosylation sites.A phylogenetic tree based on amino acid sequences displays that the goose DRD2 protein is closely related to those of avian species.Semi-quantitative RT-PCR analysis demonstrates that the DRD2-1,DRD2-2 and DRD2-4 transcripts are differentially expressed in pituitary,ovary,hypothalamus,as well as in kidney,whereas the DRD2-3 transcript is widely expressed in all the examined tissues at different levels.Meanwhile,54 single nucleotide polymorphisms(SNPs)and 4 insert-deletion(indel)variations were identified in the coding region and partial intron region of the goose DRD2 gene.Those findings will help us gain insight into the functions of the DRD2 gene in geese.
DRD2 Cloning Alternative splicing Differential expression SNPs
Cui Wang Yi Liu Huiying Wang Huali Wu Shaoming Gong Weihu Chen Daqian He
Institute of Animal Husbandry and Veterinary Science,Shanghai Academy of Agricultural Sciences,Shang Zhedong White Goose Institute of Xiangshan County,Ningbo,Zhejiang,315700,PR China
国内会议
重庆
英文
1326-1339
2016-05-25（万方平台首次上网日期，不代表论文的发表时间）