SNPs discovery and genotyping using restriction-site-associated DNA sequencing(RAD-seq)in Chickens

摘要：

　　Single nucleotide polymorphisms(SNPs)are essential to understanding of population genetic variation and diversity.Here we performed restriction-site-associated DNA sequencing(RAD-seq)on 72 individuals from 13 Chinese indigenous and 3 introduced chicken breeds.Total of 620 million reads were obtained using Illumina Hiseq2000 sequencer.We identified average 75,587 SNPs in each individual.Further filtering strictly validated 28,895 SNPs candidates for all populations.Comparing with NCBI dbSNP(chicken_9031),15,404 SNPs were new-found.With this study,we practiced RAD-seq on birds for the first time.It is implicating the remarkable effectiveness and potential applications on genetic analysis and breeding technique as the whole-genome selection in chicken and other agricultural animals.

关键词： RAD-seq SNPs discovery genotyping Chicken

作者: Z.X.Zhai W.J.Zhao C.He K.X.Yang L.L.Tang S.Y.Liu Y.Zhang Q.Z.Huang H.Meng

作者单位: Department of Animal Science,School of Agriculture and Biology,Shanghai Jiao Tong University,Shangha Department of Animal Science,School of Agriculture and Biology,Shanghai Jiao Tong University,Shangha Animal Husbandry and Veterinary Research Institute,Shanghai Academy of Agricultural Science,Shanghai Virginia Bioinformatics Institute,Virginia Tech,Washington Street,MC0477,Blacksburg,Virginia,24061;A

会议类型: 国内会议

会议名称: 第七届中国畜牧科技论坛

会议地点: 重庆

会议语种:英文

页码: 1373-1378

在线出版日期: 2016-05-25（万方平台首次上网日期，不代表论文的发表时间）