Effect of semen extender supplementation with trehalose,vitamin C and E on post-thaw min pig sperm qualities
BACKGROUND: Cryopreservation of min pig sperm cryopreservation is an effective method to protect this valuable genetic resource.OBJECTIVE: To determine whether the extender supplementation with some antioxidants is able to improve the quality of post-thawed sperm of min pig.MATERIALS AND METHODS: Ejaculated min pig sperm frozen in lactose-egg yolk(LEY)extender supplemented with trehalose,vitamin C,and/or water-soluble vitamin E were thawed,and quality of sperm were evaluated.RESULTS: The post-thawed sperm qualities were higher in the supplementation groups than that in the control group(P < 0.05).Furthermore,the addition of 100 mM trehalose and 25mM water-soluble vitamin E to the freezing extender strongly preserved the quality of the sperm(P < 0.05).CONCLUSION: Trehalose and water-soluble vitamin E in the freezing extender effectively improved post-thaw qualities of min pig sperm.
sperm quality cryopreservation min pig
Hong Ma Di Liu Wentao Wang Liang Wang Bo Fu Zhongqiu Li Xinmiao He
Heilongjiang Academy of Agricultural Sciences,Harbin,150086,China
国内会议
重庆
英文
1433-1438
2016-05-25（万方平台首次上网日期，不代表论文的发表时间）