Piwil1Mediates Meiosis during Spermatogenesis in Chicken
Piwil1 mediates spermatogenesis and ensures stable cell division rates in germline cells in mammals.However,the involvement of Piwil1 in poultry spermatogenesis and meiosis is poorly understood.In the present study,we used TaqMan RT-qPCR to characterize Piwil1 mRNA expression in different types of spermatogenic cells,including primordial germ cells(PGCs),spermatogonial stem cells(SSCs),spermatogonia cells(Sa),tetraploid cells(Tp),round sperm cells(Rs),mature sperm,and in PGCs treated with retinoic acid.Our results revealed that Piwil1 is differentially expressed during spermatogenesis in chicken.Compared to PGCs,SSCs,Tp,and Sa,Rs cells presented the highest Piwil1 mRNA expression levels.Retinoic acid significantly upregulated Piwil1 and Stra8 mRNA expression as well as Piwil1 for protein levels in chicken PGCs.In addition,retinoic acid induced PGCs to progress through all the meiotic stages,eventually leading to haploid cell formation,which was determined using flow cytometry and western blot analysis.Taken together,our results showed that during spermatogenesis,Piwil1 was first expressed at low levels in germ stem cells,PGCs,and SSCs.Its expression levels increased during later meiosis stages.Finally,no expression was detected in mature sperm after meiosis.Treatment of PGCs with retinoic acid further demonstrated that Piwil1 plays a key role in meiosis during chicken spermatogenesis.
chicken Piwil1 meiosis spermatogenesis germ cells
Lu Xu Guobin Chang Teng Ma Hongzhi Wang Jing Chen Zhiteng Li Xiaomin Guo Fang Wan Lichen Ren Wei Lu Guohong Chen
Jiangsu Key Laboratory for Animal Genetic,Breeding and Molecular Design,Yangzhou University,Yangzhou 225009,Jiangsu,China
国内会议
重庆
英文
1439-1452
2016-05-25（万方平台首次上网日期，不代表论文的发表时间）