Dynamical Expression of MicroRNA-127-3p In Proliferating and Differentiating C2C12 Cells

摘要：

　　Background MicroRNAs(miRNAs)are highly conserved,short non-coding RNAs that regulate gene expression at the posttranscriptional level.Although many miRNAs are identified in muscles and muscle cells,their individual roles are still not fully understood.Result In the present study,we investigated a muscle highly-expressed miRNA,miR-127-3p,in C2C12 myoblasts and tissues of goats with different muscle phenotypes(Boer vs.Wushan black goats).Our results demonstrated that(1)miR-127-3p was extensively expressed in tissues of goats;(2)miR-127-3p was higher expressed in muscle,spleen,heart and skin in the muscular goats(Boer goats)than the control(the Wushan black goats).Then we further characterized the dynamical expression of miR-127-3p,MyoD,MyoG,Myf5,Mef2c and Myosin in the proliferating and differentiating C2C12 myoblasts at day of 0,1,3,5,and 7 in culture mediums.Especially,we found that miR-127-3p was significantly higher expressed in the proliferating than differentiating cells.Conclusion Our findings suggest that miR-127-3p probably play roles in the proliferation and differentiation of myoblasts,which further underlies regulation of muscle phenotype in goats.

关键词： MiR-127-3p C2C12 Proliferation Differentiation Goats

作者: Jie li Gaofu Wang Jing Jiang Peng Zhou Liangjia Liu Jinhong Zhao Lin Wang Yongfu Huang Youji Ma Hangxing Ren

作者单位: College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,Gansu,China;Ch Chongqing Academy of Animal Science,Rongchang 402460,Chongqing,China Chongqing Academy of Animal Science,Rongchang 402460,Chongqing,China;College of Animal Science and T College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,Gansu,China

会议类型: 国内会议

会议名称: 第七届中国畜牧科技论坛

会议地点: 重庆

会议语种:英文

页码: 1490-1498

在线出版日期: 2016-05-25（万方平台首次上网日期，不代表论文的发表时间）