Controlled delivery of GHRP-6 from PLGA-PEG-PLGA copolymer and the effect of GHRP-6-copolymer hydrogel on the growth of rex rabbits
(0) Growth hormone releasing peptide-6(GHRP-6)plays an important role in animal growth.However,there was few study focused on the effect of GHRP-6 on animal growth through controlled-release system.We have synthesized PLGA-PEG-PLGA copolymer to investigate its control-released effect on GHRP-6 in vitro and to study the effect of GHRP-6-copolymer hydrogel on the growth of rex rabbits.The copolymer was synthesized using ring-opening copolymerization and characterized by 1H NMR.The interaction between GHRP-6 and copolymer was characterized by Fourier transform infrared(FTIR)and X-ray diffraction(XRD).The body weight,serum level of insulin-like growth factor-1(IGF-1)and hair coat quality were studied in rex rabbits.The results showed that hydrogen bonding forms between N-H group in GHRP-6 and C=O group in copolymer.The release mechanism of GHRP-6 was the combination of diffusion-controlled and erosion-controlled in the copolymer.The serum level of IGF-1,hair coat quality and body weight was both significantly higher in the GHRP-6-copolymer hydrogel group than other groups.These results indicate that the copolymer can effectively control the release of GHRP-6.In addition,the GHRP-6-copolymer hydrogel can increase the synthesis of IGF-1 for a prolonged period,thereby increasing the rex rabbits growth and hair coat quality.
Yuan Guan Xinwei Li Xin Zhang Yunyun Cheng Dan Su Chao Lu Xibi Fang Qiang Ma Dawei Zhang Hao Yu Linlin Hao Songcai Liu
College of Animal Science,Jilin University,5333 Xi”an Road,Changchun,Jilin 130062,China College of Veterinary Medicine,Jilin University,5333 Xi”an Road,Changchun,Jilin 130062,China Teaching Center of Basic Courses,Jilin University,5333 Xi”an Road,Changchun,Jilin,130062,China
国内会议
重庆
英文
1588-1601
2016-05-25（万方平台首次上网日期，不代表论文的发表时间）