Molecular cloning,characterization,and tissues expression analysis of the goose(Anser cygnoides)prepro-VIP gene
In avian,vasoactive intestinal peptide(VIP)as a prolactin(PRL)releasing factor,is involved in the control of the PRL release,and plays a pivotal role as a regulator of reproductive behavior and neuroendocrine secretion.In this study,a 833 bp fragment covering the complete coding region(CDS)of goose prepro-VIP gene was obtained.The cDNA contains a 32 bp 5′-untranslated region(UTR),a 306 bp CDS,and a 495 bp 3′-UTR including a TGA termination codon(nucleotides 518–520 bp)and the poly(A)tail.The goose prepro-VIP gene shares about 67–94%nucleic acid identity and 53–98%amino acid identity with their counterparts in other species.The sequence analysis indicated that two alternatively spliced transcripts were identified,the larger transcript(VIP-1)encoded both VIP and peptide histidine isoleucine(PHI)exons and the short one(VIP-2)only encoded VIP.Based on the cDNA sequence,the genomic structure of this gene was characterized.The goose prepro-VIP gene consists of 7 exons and 6 introns,just as that in other avian species.The RT-PCR analysis indicates that the level of the VIP-1 is much higher than that of the VIP-2,and the lager one negligible or absent in liver,kidney,muscle and abdominal fat,while the short one is widely distributed in all examined tissues.
Goose Prepro-VIP gene Molecular characterization Tissues expression profile
Yi Liu Cui Wang DaQian He HuiYing Wang HuaLi Wu
Institute of Animal and Veterinary Science Research,Shanghai Academy of Agricultural Sciences,Shanghai,201106 China
国内会议
重庆
英文
1622-1634
2016-05-25（万方平台首次上网日期，不代表论文的发表时间）