Separation of a minor lignin from Sinopodophyllum emodi by MPLC and HSCCC guided by HPLC-MS
A minor lignin,4”-demethyldeoxypodophyllotoxin,was isolated from Sinopodophyllum emodi.First,a crude extract of Sinopodophyllum emodi was analyzed by HPLC-MS to confirm the presence of the target compound.Then,the target lignin was successfully enriched in the crude extract precipitate by medium-pressure LC with high recovery(85%).Finally,an efficient separation was achieved by high-speed counter-current chromatographyusing a two-phase solvent system of n-hexane-ethyl acetate-methanol-water(1:1:1:1,v/v/v/v).These results clearly demonstrate that the combination of HPLC-MS,medium pressure LC and high-speed counter-current chromatography could be a powerful technique for the rapid screening,enrichment and separation of minor compounds from complex natural products.
Sinopodophyllum emodi high-speed counter-current chromatography HPLC-MS 4”-demethyldeoxypodophyllotoxin minor lignin
Tao Chen Hongmei Li Liang Tan Yulin Li
Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining,810001 Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining,810001;University of the C
国内会议
成都
英文
51-56
2017-03-30（万方平台首次上网日期，不代表论文的发表时间）