Accurate quantification of exo-and endogenous blood glucose by isotope dilution LC-MS/MSwith indirect MRM of the derivative tag
Liquid chromatography coupled to tandem mass spectrometry(LC-MS/MS)is a highly specific and sensitive techniqueformeasuring metabolites.In this work,a method using thederivatization reaction combined withisotope dilutionLC-MS/MSanalysis to accurately quantify exogenous and endogenousglucose in plasma was developed.The mass spectrometer was carried outin multiple reaction monitoring mode(MRM)with electrospray ion source(ESI).Validation results indicated this method was robust,highly sensitive and significant improvement the accuracy of glucose quantification.Thecalibration curveshowed good linearity with correlation coefficient ofR2=0.9967 and the relative standard deviation(RSD%)calculated from triplicate analyses for each sample wasalways <2.5%.
LC-MS/MS endogenous and exogenous glucose MRM
Lingling Yu Chao Wen Xing Li Shiqi Fang Kaifeng Hu
State Key Laboratory of Phytochemistry and Plant Resources in West China,Kunming Institute of Botany State Key Laboratory of Phytochemistry and Plant Resources in West China,Kunming Institute of Botany State Key Laboratory of Phytochemistry and Plant Resources in West China,Kunming Institute of Botany
国内会议
成都
英文
141-142
2017-03-30（万方平台首次上网日期，不代表论文的发表时间）