Exosomes from High Glucose-Treated Glomerular Endothelial Cells Activate Mesangial Cells to Promote Renal Fibrosis
The interaction between glomerular endothelial cells (GECs) and glomerular mesangial cells (GMCs) is an essential aspect of diabetic nephropathy (DN).Therefore, understanding how GECs communicates with GMCs in the diabetic environment is crucial for the development of new targets for the prevention and treatment of DN.Exosomes, nanometer-sized extracellular membrane vesicles secreted by various cell types, play important roles in cell-to-cell communication via the transfer of mRNA, microRNA and protein.In this study, we demonstrate that high glucose (HG)-treated GECs secret a higher number of exosomes highly enriched in TGF-1 mRNA compared with normal glucose (NG)-treated GECs.Exosomes released by HG-treated GECs can promote-smooth muscle actin (-SMA) expression, proliferation, extracellular matrix protein overproduction in GMCs through TGF-1/Smad3 signaling pathway.Thus, we provide new insights into the pathogenesis of DN that involves intercellular transfer of TGF-1 mRNA in the GECs-GMCs direction via exosomes.
Exosome Glomerular endothelial cells Glomerular mesangial cells High glucose Activation Renal Fibrosis
Xiao-ming Wu Yan-bin Gao Fang-qiang Cui Na Zhang
Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, No.10, Youanmenwai, Xitoutiao,Fengtai District, Beijing 100069, China
国内会议
北京
英文
76-92
2016-11-01（万方平台首次上网日期，不代表论文的发表时间）